Diabetik Sıçanlarda Melatonin Uygulamasının Karaciğer, Böbrek, Mide, Pankreas ve Göz Dokularında Oksidatif Stres Üzerine Etkisi

Meryem Ergenç, Salim Özenoğlu, İnci Turan, Veysel Haktan Özaçmak, Hale Sayan Özaçmak

Öz


Oksidatif stresin diabetik komplikasyonların gelişiminde çok önemli rol oynadığı yaygın olarak kabul edilmektedir. Böylece koruyucu tedaviler hastalığın olası yan etkilerini hafifletebilir. Bu çalışmanın amacı melatonin tedavisinin diabetik sıçanların karaciğer, böbrek, pankreas, mide ve retinal dokularındaki oksidan ve antioksidan durumuna etkisini incelemektir.
Erkek Wistar albino cinsi sıçanlar 4 gruba ayrıldılar: Kontrol, kontrol+melatonin, diabetik ve diabet+melatonin uygulanan grup. Sıçanlarda intraperitoneal (i.p) olarak tek doz streptozotosin (STZ) (60 mg/kg) uygulaması ile diabet oluşturulmuştur. Melatonin 10 mg/kg dozunda günde tek doz i.p olarak 30 gün boyunca uygulanmıştır. 30 günlük tedavinin sonunda indirgenmiş glutatyon (GSH) ve malondialdehid (MDA) düzeyleri karaciğer, böbrek, pankreas, mide ve retinada ölçülmüştür.
Sonuçlarımız diabetik hayvanların dokularında MDA düzeylerinin arttığını ve GSH seviyelerinin azaldığını göstermiştir. Melatonin uygulaması karaciğer dışındaki dokularda MDA düzeylerini anlamlı olarak düşürmüştür. Melatonin tedavisi diabet grubuna göre karşılaştırıldığında GSH düzeylerinde pankreas ve mide dışındaki dokularda artış bulunmuştur.
Bu çalışmada sunulan sonuçlar, diyabete, diyabetik komplikasyonların başlıca nedeni olarak kabul edilen oksidatif stresin eşlik ettiği ortak görüşüyle uyumludur.
Sonuçlarımız melatoninin diyabette artan oksidatif stres üzerinde iyileştirici etkisi ile kullanılabileceğini göstermektedir.

Anahtar Kelimeler


Diabet; melatonin; oksidatif stres; indirgenmiş glutatyon

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Referanslar


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